Preclinical Bone Growth Research

Recent Preclinical Research. The cited 2020 and 2025 rat studies measured longitudinal bone growth rate, femur and tibia length, growth plate histology, bone microarchitecture, and IGF 1 related signaling after H. japonicus exposure.

Study Conditions. The cited bone growth experiments used growing rat models, defined dose groups, and specified treatment periods. Outcomes were analyzed across those experimental groups.

• Pathway Measurements. The studies measured IGF 1 and growth hormone related markers together with JAK2/STAT5/IGF 1 pathway activity and skeletal endpoints.
• Study Data and Patent Reference. In the cited controlled studies, investigators compared longitudinal bone growth rate, femur and tibia length, and micro CT trabecular measurements across dose groups and a growth hormone control group. The page also lists U.S. Patent Application No. 18/456,888, which describes a Humulus japonicus composition for height growth applications.
• Growth Stage Variables. The cited preclinical studies recorded age and growth stage as experimental variables when evaluating longitudinal bone growth outcomes.

Additional Research Areas

Additional studies of Humulus japonicus report measurements related to oxidative stress signaling, inflammatory pathways, respiratory endpoints, microbial activity, metabolism, gastrointestinal physiology, and tyrosinase activity.

• AMPK/SIRT1 and Oxidative Stress Markers. Sung et al. (2015) measured AMPK/SIRT1 pathway activity and oxidative stress related endpoints in its experimental model.
• Inflammatory Signaling Research. Studies of Humulus japonicus flavonoids and phenolic compounds report cytokine and inflammatory pathway measurements.
• Bioactive Compound Research. The cited literature examines respiratory, neurological, liver, antimicrobial, metabolic, gastrointestinal, and tyrosinase related endpoints. The SNEDDS section separately summarizes formulation measurements relevant to delivery of lipophilic compounds.

Care, Usage, and Formulation

• Do NOT: Do not refrigerate. Do not place near the sun or in super warm liquids. Do NOT shake if the product is already diluted.
• You CAN: Take Japonicus by itself or put it in water to drink. You can drink other beverages before or after taking it.
• Timing. Use the labeled serving directions and a consistent measurement routine.
• Taste Profile. The formulation contains no sugary flavoring. Taste responses vary, particularly with the formulation excipients.

Measurement and Serving Guide

Measurement Guide. The marked dropper measures the serving in milliliters. The labeled range shown on this page begins at 0.5 mL and extends to 2.0 mL.

How long will your bottle last?

Note: 10 drops is approximately 0.5 mL. Max recommended dose is 2mL/day.

Research References

• Kim OK, Yun JM, Lee M, Park SJ, Kim D, Oh DH, Kim HS, Kim GY. A Mixture of Humulus japonicus Increases Longitudinal Bone Growth Rate in Sprague Dawley Rats. Nutrients. 2020 Aug 28;12(9):2625.
• Park SH, Park J, Yoo JY, Kim HS, Lee M, Kim OK. Humulus japonicus Enhances Bone Growth and Microarchitecture in Rats: Potential Involvement of IGF-1 Signaling. J Med Food. 2025 Jun;28(6):542-552.
• Kim, O. K., et al. (2023). Food composition for promoting height growth and pharmaceutical composition for promoting height growth including Humulus japonicus extract. U.S. Patent Application No. 18/456,888. Washington, DC: U.S. Patent and Trademark Office.
• Sung B, Chung JW, Bae HR, Choi JS, Kim CM, Kim ND. Humulus japonicus extract exhibits antioxidative and anti-aging effects via modulation of the AMPK-SIRT1 pathway. Exp Ther Med. 2015 May;9(5):1819-1826. doi: 10.3892/etm.2015.2302. Epub 2015 Feb 17. PMID: 26136899; PMCID: PMC4471809.